Development of Loop Mediated Isothermal Amplification (LAMP) Assay for Detection of PCV2
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چکیده
منابع مشابه
Development of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Sensitive Identification of Ostrich Meat
Animal species identification is one of the primary duties of official food control. Since ostrich meat is difficult to be differentiated macroscopically from beef, therefore new analytical methods are needed. To enforce labeling regulations for the authentication of ostrich meat, it might be of importance to develop and evaluate a rapid and reliable assay. In the present study, a loop-mediated...
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Bacillus cereus is a facultative anaerobic, spore-forming, motile microorganism that can be commonly isolated from food. It is known to produce four toxins, one emetic toxin and three potential diarrheal toxins (Ehling-schulz, et al., 2004; Samapundo, et al., 2011). The emetic syndrome is mainly characterized by vomiting 0.5 6 h after ingestion of the contaminated food. In the diarrheal syndrom...
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Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
متن کاملElectric LAMP: Virtual Loop-Mediated Isothermal AMPlification
We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole ...
متن کاملdevelopment and assessment of loop-mediated isothermal amplification (lamp) assay for the diagnosis of human visceral leishmaniasis in iran.
background: parasitological methods for the diagnosis of visceral leishmania-sis (vl) require invasive procedures, so serological and molecular approaches have been developed but are not generally applicable in the field. we evaluated a loop mediated isothermal amplification (lamp) assay using blood from vl pa-tients and compared it to nested pcr. methods: forty-seven subjects with clinical fea...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2020
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2020.904.288